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Dec 22, 2024
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BIOT 275 BIOTECHNOLOGY C-NUCLEIC ACIDS4 Units - 3 hours lecture, 3 hours lab
Prerequisite: BIOT 70 or BIO 1 or BIO 16 or BIO 82
Nucleic acids are organic molecules that allow organisms to transfer genetic information from one generation to the next. This course provides students with advanced laboratory skills needed for employment in the biotechnology industry. Students will be re-introduced to the basic concepts of molecular biology including DNA structure and function as well as the process and controls of gene expression. Techniques include DNA isolation, purification and sequencing, gel electrophoresis, fluorescence in situ hybridization and Southern Blot analysis, DNA restriction/fingerprinting, cloning (transformation and screening) and real-time PCR. Maintenance of an industry standard notebook, application of SOP and instrument maintenance and care emphasized. Students will also be introduced to the field of bioinformatics. NR
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