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    Mar 28, 2024  
2020-2021 Catalog 
    
2020-2021 Catalog [ARCHIVED]

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BIOT 77 BIOTECHNOLOGY: CELL CULTURE

2 Units - 1 hour lecture, 3 hours lab
Transfers: CSU, UC
Prerequisite: BIO 10    and BIO 15  
This course provides theoretical and practical knowledge and skills on how to culture Eukaryotic cells. Students acquire practical and theoretical knowledge of the structure, equipment and sterile techniques of the cell culture laboratory, the growth conditions of cells and how scientists attempt to mimic this in cultures. Among the topics covered are: the composition of cell culture media, establishment of primary cultures and cell lines from normal tissue and cancer tissue, routine cultivation of cells, long term storage, contamination, various methods for characterization of cells, transfection, and the use of cells in culture to resolve various issues in basic and applied research.  NR



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